How students should use this module
1
Read the lab introduction
Review pteridine and ommochrome pathways, transporter genes, eye-color mutants, and epistasis.
2
Score F₂ flies
Observe your vial, identify sex and phenotypes, record data, then discard the vial as instructed.
3
Set up the TLC experiment
Work in groups, collect five eye-color phenotypes, extract pigments, spot the TLC plate, and run the chromatography.
4
Use the Pigment Pathway Simulator
Create the assigned genotypes and connect pigment presence or absence to eye color phenotypes.
5
Submit the assignment
Complete Assignment #5 and submit a physical copy or scanned PDF through Canvas.
Module links
These buttons are the same resources shown in Canvas, but organized as a single Lab 5 landing page.
Suggested workflow:
1. Score your F₂ flies in the laboratory.
2. Extract eye pigments and set up the TLC plate.
3. Use the Pigment Pathway Simulator to test genotypes.
4. Complete and submit Assignment #5.
1. Score your F₂ flies in the laboratory.
2. Extract eye pigments and set up the TLC plate.
3. Use the Pigment Pathway Simulator to test genotypes.
4. Complete and submit Assignment #5.
Lab Instructions
Open in new tabAssignment #5
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