How students should use this module
1
Read the molecular genetics background
Review the white-1 locus, Doc-retroposon insertion, sex-linked inheritance, PCR, and gel electrophoresis.
2
Perform DNA extraction
Work in groups, extract DNA from one fly type, measure sample concentration and purity, and record the values.
3
Set up the PCR reaction
Review the PCR cycler program, select the most suitable conditions, and prepare the reaction as instructed by the TAs.
4
Use the Virtual PCR Tool
Upload the five DNA sequence files, enter PF1, PF2, and PR3, run the required PCRs, and record fragment sizes.
5
Interpret PCR and gel results
Use 467 bp and 704 bp fragments to infer genotypes, phenotypes, heterozygotes, and expected gel bands.
6
Submit the assignment
Complete Assignment #8 and submit a physical copy or scanned PDF through Canvas.
Module links
These buttons are the same resources shown in Canvas, but organized as a single Lab 8 landing page.
Suggested workflow:
1. Complete the in-person DNA extraction and record DNA quality values.
2. Review the PCR cycler program and choose the correct PCR conditions.
3. Use the Virtual PCR Tool with PF1, PF2, and PR3 to genotype the five DNA sequences.
4. Use the predicted fragments to complete the gel templates and genotype table.
5. Complete and submit Assignment #8.
1. Complete the in-person DNA extraction and record DNA quality values.
2. Review the PCR cycler program and choose the correct PCR conditions.
3. Use the Virtual PCR Tool with PF1, PF2, and PR3 to genotype the five DNA sequences.
4. Use the predicted fragments to complete the gel templates and genotype table.
5. Complete and submit Assignment #8.
Lab Instructions
Open in new tabAssignment #8
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